Proteomic Methodologies to Assess Modifications
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- Product Stewardship for Sustainability Certificate Program
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- Apply
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- EHE Research Retreat
- Faculty Research Interests
- Dr. Kirsten Koehler Faculty Research
- Aerosol Samplers
- Baltimore Healthy Schools: Impact of Indoor Air Quality on Health and Performance
- Exposure Assessment for Epidemiologic Studies
- Exposures from Oil Spills
- Kirsten Koehler's Research Team
- Occupational Hazard Mapping
- Particulate Exposures in Asthmatic Kids (PEAK)
- Personal Exposure Assessment
- Publications
- Spatiotemporal Exposure Assessment
- Within Baltimore Variability in Pollution
- Kohr Laboratory of Cardiovascular Redox Signaling
- One Health Laboratory at Johns Hopkins University
- Animal Visitation Chlorhexidine Trial
- Baltimore Healthy Schools: Impact of Indoor Air Quality on Health and Performance
- Collaborators
- Dr. Meghan Davis Receives Canine Health Foundation Grant to Study the Health of Dogs
- Group on the Integration of the Relationship between Animals, Public Health and Ethics
- News and Publications
- One Health Research Team
- The COVET Study
- The CoWelf Study
- The Minority Pet-Owner Health Project
- Zoonotic Spillover Projects
- COVID-19 Human-Animal Interactions Survey (CHAIS)
- Research Program Gurumurthy Ramachandran, PhD
- Climate Change, Pesticide Use, and Exposures
- Computed Tomography and Low-cost Sensor Networks to Reconstruct Spatial Pollutant Distributions
- Decision-making for Risk Management Using Small Data Sets, Mathematical Models, and Heuristics
- Infrastructure for Delivering and Characterizing Airborne Exposures in Exposure Chambers
- Mathematical Modeling of Chemical Exposures
- Meet Ram's Team...
- Modeling of Infectious Diseases
- The Exposome Collaborative @ Johns Hopkins University
- The Johns Hopkins Environmental Health Microbiology and Immunology Laboratory
- Dr. Kirsten Koehler Faculty Research
- Geyh-Bouwer Trainee Practice Award
- Grand Rounds
- Mobile Air Pollution Measurement Laboratory
- Research Areas
- Air Pollution and Cardiorespiratory Diseases
- Antimicrobial Resistance and Infectious Disease
- Biosecurity and Emerging Threats
- COVID-19 Research
- Carcinogens and Cancer
- Children's Environmental Health
- Chronic Disease Etiology and Prevention
- Community Sustainability, Resilience, and Preparedness
- Consumer Product Safety
- Energy Management and Alternative Technologies
- Environmental Chemistry, Microbiology and Ecology
- Environmental Engineering
- Environmental Epidemiology
- Environmental Inequities and Injustice
- Environmental Resource Quality
- Epigenetic Regulation in Environmental Diseases
- Food and Agricultural Systems
- Geomorphology, Geochemistry, and Hydrology
- Global Environmental Change
- Novel Exposure Assessment
- Risk Sciences and Public Policy
- Social and Behavioral Sciences
- Toxicology, Physiology, & Cell Biology
- Water, Sanitation, Hygiene, and Health
- Worker Health and Safety
- Teaching and Research Labs at WSE
- The INnovations to Generate Estimates of children's Soil/dust inTake (INGEST) Study
- Centers and Institutes
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Our laboratory has developed a number of novel gel-based and gel-free proteomic approaches for examining redox-based post-translational protein modifications. As such, we were the first to map specific in situ S-nitrosation and oxidation sites and occupancy levels in the heart at baseline, with cardioprotection, and following ischemia-reperfusion injury (Kohr et al. 2011, Kohr et al. 2011, Kohr et al. 2012).
Our studies utilized these novel proteomic approaches to demonstrate the ability of S-nitrosation to shield critical thiols from the damaging effects of irreversible oxidation, thus leading to the preservation of cellular function and viability. These seminal findings represent important advances toward the development of therapies for disease states where oxidant stress is a critical component of pathogenesis (i.e., ischemic heart disease, diabetes, Parkinson's disease, Alzheimer's disease). These studies also represent important advances in the biology of S-nitrosation, and demonstrate the potential for significant contributions to cellular physiology and function.
We continue to develop novel methodologies to further characterize redox-based modifications in the heart. Current studies are focused on the development of proteomics-based methods to characterize crosslinks induced by the endogenous production of formaldehyde in the heart, especially in the context of ischemia-reperfusion injury. These approaches are critical for defining the role of redox signaling in cardioprotection and ischemic injury.