Study: Toxic Metals Found in E-Cigarette Liquids

Five metals, toxic and potentially carcinogenic when inhaled, found in liquid of leading first generation e-cigarette brands

A study led by researchers at the Johns Hopkins Bloomberg School of Public Health found high levels of toxic metals in the liquid that creates the aerosol that e-cigarette users inhale when they vape.

The study, believed to be the first to examine a cross-section of metals in multiple e-cigarette brands, analyzed the liquid in five brands of first generation e-cigarettes for cadmium, chromium, lead, manganese and nickel. The liquid is the component of e-cigarettes that, when heated, delivers ingredients, often including nicotine and flavors, to the user. In first generation e-cigarettes, the liquid is stored in the cartridge in close contact with the heating coil. The researchers found all five heavy metals – which can be toxic or carcinogenic when inhaled – in all five brands, though levels varied by brand. The main source of the metals, the researchers believe, is the coil that heats the liquid that creates the aerosol, which is often but erroneously referred to as vapor. The study did not look at the possible presence of metals in e-cigarette aerosol.

The findings appear in the January issue of the journal Environmental Research.

“We do not know if these levels are dangerous, but their presence is troubling and could mean that the metals end up in the aerosol that e-cigarette users inhale,” says study leader Ana María Rule, PhD, MHS, an assistant scientist in the Bloomberg School’s Department of Environmental Health and Engineering. “One of the things that is troubling is that the metals in e-cigarette coils, which heat the liquid that creates the aerosol, are toxic when inhaled, so perhaps regulators might want to look into an alternative material for e-cigarette heating coils.”   

The Food and Drug Administration began regulating e-cigarettes last year, but has not yet issued warnings. E-cigarettes may be less harmful than cigarettes for current smokers who switch completely to electronic cigarettes. A serious concern is the appeal of e-cigarettes to young people who have never smoked, since e-cigarettes might be habit forming, and might not be totally safe as emerging research shows that nicotine can adversely affect the developing adolescent brain. Last fall, then-U.S. Surgeon General Vivek Murthy called e-cigarette use by young people a serious concern. E-cigarette use among high school students jumped 900 percent between 2011 and 2015.

For their study, the researchers selected five leading brands of so-called first generation e-cigarettes, which are referred to as cig-a-likes because they resemble traditional cigarettes. (Newer ones look like small cassette recorders with a mouthpiece. In the newer devices the liquid is added from a dispenser prior to use. In contrast, the liquid in first generation e-cigs is stored in the cartridge together with the coil, which increases the liquid’s exposure to the coil even in the absence of heating.) The five brands are sold across the United States in big-box retail stores, convenience stores and gas stations, as well as online. Three of the five brands constituted 71 percent of total market share in 2015. If a brand came in more than one flavor, the researchers chose one flavor for consistency’s sake.

To test the liquid for metal levels, the researchers extracted samples of the liquid; the liquid had not been heated by the coil prior to extraction. The liquid is a mixture of propylene glycol, glycerin, nicotine and flavorings. Because liquid volume varied considerably from brand to brand, the research team tested for concentrations of metals in micrograms per liter.

The five metals – cadmium, chromium, lead, manganese and nickel – were present in all five brands, with cadmium markedly lower than the other metals and with a considerable range of concentrations among the brands. For instance, one brand had a high concentration of all five metals. In that brand, the concentration of nickel, which is considered the most serious carcinogen when inhaled, was 22,600 micrograms per liter, 400 times that of the brand with the lowest concentration of nickel. In that same brand, the one with the highest concentration of all five metals, the concentration of manganese was 690 micrograms per liter, or 240 times that of the lowest concentration in yet another brand.

“It was striking, the varying degrees to which the metals were present in the liquid,” Rule says. “This suggests that the FDA should consider regulating the quality control of e-cigarette devices along with the ingredients found in e-cigarette liquids.”

For now, FDA regulations require e-cigarette makers to submit ingredient lists as well as information about potentially harmful ingredients, including four of the five metals analyzed in this study – nickel, lead, chromium and cadmium. The FDA has yet to issue proposed regulations on e-cigarette labeling. In addition to the coil, the researchers believe some of the metals may come from the components of the e-cigarette device or the manufacturing process.

“E-cigarettes as a source of toxic and potentially carcinogenic metals” was written by Catherine Ann Hess, Pablo Olmedo, Ana Navas-Acien, Walter Goessler, Joanna E. Cohen and Ana María Rule.

This study was funded by the Institute for Global Tobacco Control, Johns Hopkins School of Public Health; National Institute of Environmental Health Sciences Training Grant T32ES007141-31A1; National Institute on Alcohol Abuse and Alcoholism Training Grant T32-AA014125 and the Alfonso Martín Escudero Foundation.

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Media contacts: Barbara Benham at 410-614-6029 or bbenham1@jhu.edu and Stephanie Desmon at 410-955-7619 or sdesmon1@jhu.edu.